Trump's ultimatum on Friday puts pressure on Europeans - key backers and parties to the 2015 global agreement to curb Iran's nuclear program - to satisfy Trump, who wants the pact strengthened with a separate agreement within 120 days.
It also said Iran would not allow the deal to be linked to other issues, after Mr Trump suggested that the sanctions relief under the deal be tied to Iran limiting its long-range ballistic missile program.
"This is a last chance".
Iran says its nuclear programme is only for peaceful purposes.
Trump has come under heavy pressure from European allies to issue the sanctions waiver.
He also said USA law must tie long-range missile and nuclear weapons programmes together, making any missile testing by Iran subject to "severe sanctions".
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Iranian Foreign Minister Mohammad Javad Zarif has criticized President Trump, saying the nuclear deal is not renegotiable. However, it is worthy of note that all these areas that Iranians are complaining about are outside the scope of the nuclear deal.
Additionally, the president is hoping that the Iran deal will require better United Nations inspections of Iran's nuclear sites and that Iran avoid a "breakout period" of being able to produce enough uranium for a nuclear bomb.
The U.S. possible decision to quit the nuclear deal with Iran will affect strengthening of the non-proliferation regime, the diplomat said, while commenting on a recent statement by the U.S. President Donald Trump.
Under the deal, Iran agreed to curb its nuclear program, in exchange for the lifting of most sanctions.
The remark comes on the heels of Trump's decision on Friday to sign a waiver on Iranian sanctions, suspending punitive measures for another 120 days and keeping the Iran nuclear deal alive.
Trump has repeatedly denounced the nuclear agreement negotiated by his predecessor and in October declined to certify that it's in the interests of the US.
"We have sanctioned almost 100 individuals and entities involved with the Iranian regime's ballistic missile program and its other illicit activities". Bhala can discuss why the president's position is wrong as well as the requirements of the deal for the U.S., Iran and global allies.
He accused Iran of "not living up to the spirit" of the agreement even though the International Atomic Energy Agency says Tehran is complying.