"President Daniel Ortega, abandon your arrogant attitude, listen to the people, embrace dialogue with sincerity, feel the pain of so many families and contribute to peace in the country", he tweeted.
On Monday police were kept back from demonstrators, who reportedly shouted "Murderers!"
The U.S. government stripped its embassy in Nicaragua down to bare-bone operations Monday after five days of deadly protests around the country, despite Nicaraguan President Daniel Ortega's efforts to calm his tempestuous nation.
Police were criticised for their heavy-handed response to the protests, and accused of using live ammunition against demonstrators.
While Mr. Ortega said Sunday that he was withdrawing the proposed changes, the unrest has continued.
La Prensa, a conservative paper that has been a harsh critic of both Ortega and the previous regime he fought to overthrow, said he had "lost control of the streets".
Guido said the Red Cross had registered nine deaths since the protests began Wednesday, and attended to 433 injured people.
"We are particularly concerned that a number of these deaths may amount to unlawful killings", U.N. High Commissioner For Human Rights spokeswoman Liz Throssel said Tuesday.
U.S. Ambassador Michael Kozak, the senior official in the State Department's Bureau of Democracy, Human Rights, and Labor said last week that the Nicaraguan election was "a sham" and asserted the country "is going in the wrong direction on many fronts".
A State Department official said the embassy in Managua would shutter many of its operations until further notice but that it would continue to provide services to USA citizens and visa applicants. But despite criticism of the overhaul coming from business leaders, university students and elderly pensioners, the president publicly blamed right-wing groups for the inciting violence.
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